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Idaho State University

Idaho State University, a Carnegie-classified doctoral research and teaching institution founded in 1901, attracts students from around the world to its Idaho campuses. At the main campus in Pocatello, and at locations in Meridian, Idaho Falls and Twin Falls, ISU offers access to high-quality education in more than 250 programs.

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Data from: DNA methylation of two heat-stress genes in redband trout from contrasting thermal environments

Dataset includes supporting environmental data for sequencing data submitted to NCBI SRA database BioProject PRJNA1016365, and utilized within the following peer-reviewed manuscript in [Conservation Genetics](###Pending Review###).

Epigenetic variation is a potential pathway for rapid response to environmental change and may influence local adaptation at the population level through population by environment interactions. Here, we focus on the most well-understood mechanism of epigenetic variation, DNA-methylation. Previous lab studies have shown consistent ecotypic variation in DNA-methylation, but few have attempted to quantify epigenetic variation in natural populations. We developed a study to compare levels of DNA-methylation in two heat-stress related genes in redband trout (Oncorhynchus mykiss gairdneri) from contrasting environments. We sampled trout populations from cold montane and warm desert streams at repeated intervals, collecting tissue samples that were used for epigenetic analysis and levels of DNA-methylation (percent methylation) were quantified using targeted bisulfite sequencing. There were no differences among fish from contrasting ecotypes in methylation at heat shock protein 70 (HSP70) or heat shock protein 47 (HSP47), indicating that seasonal changes in DNA methylation may occur at finer spatial scales than at the level of ecotype. However, there was significant variation in DNA methylation level between montane populations at heat shock protein 47. These findings suggest that DNA methylation is unlikely to act as a source of plastic adaptive phenotypic variation in natural redband trout populations and therefore may not contribute greatly to thermal adaptation in coldwater fishes. Further research is needed to better inform whether and when DNA methylation might act as a of adaptive phenotypic variation, and more specifically, how population-level adaptation may influence epigenetic change in wild populations.

Data Use
License: Attribution 4.0 International (CC-BY 4.0)
Recommended Citation:
Kline BC, Williams BR, Loxterman JL, Keeley ER. 2023. Data from: DNA methylation of two heat-stress genes in redband trout from contrasting thermal environments [Dataset]. University of Idaho.

US National Science Foundation and Idaho EPSCoR: OIA-1757324

Release Date
Spatial / Geographical Coverage Area
POLYGON ((-116.952848 42.548799, -116.952848 44.5556, -116.025399 44.5556, -116.025399 42.548799))
Spatial / Geographical Coverage Location
southwestern Idaho USA
Temporal Coverage
Friday, June 5, 2020 - 00:00 to Saturday, October 24, 2020 - 00:00
English (United States)
Benjamen Kline, Brigette R Williams, Janet L Loxterman, Ernest R Keeley
Contact Name
Benjamen Kline
Contact Email
Public Access Level
Data available on:: 
Sunday, June 30, 2024