{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"240f0f19-3151-4779-9773-22bff99f6674","name":"data-dna-methylation-two-heat-stress-genes-redband-trout-contrasting-thermal-environments","title":"Data from: DNA methylation of two heat-stress genes in redband trout from contrasting thermal environments","author":"Benjamen Kline, Brigette R Williams, Janet L Loxterman, Ernest R Keeley","author_email":"klineben@msu.edu","maintainer":"RCDS Data Repository","maintainer_email":"rcds-web@uidaho.edu","license_title":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/","notes":"\u003Cp\u003EDataset includes supporting environmental data for sequencing data submitted to NCBI SRA database BioProject PRJNA1016365, and utilized within the following peer-reviewed manuscript in [\u003Cem\u003EConservation Genetics\u003C\/em\u003E](###Pending Review###).\u003C\/p\u003E\n\u003Cp\u003EEpigenetic variation is a potential pathway for rapid response to environmental change and may influence local adaptation at the population level through population by environment interactions. Here, we focus on the most well-understood mechanism of epigenetic variation, DNA-methylation. Previous lab studies have shown consistent ecotypic variation in DNA-methylation, but few have attempted to quantify epigenetic variation in natural populations. We developed a study to compare levels of DNA-methylation in two heat-stress related genes in redband trout (Oncorhynchus mykiss gairdneri) from contrasting environments. We sampled trout populations from cold montane and warm desert streams at repeated intervals, collecting tissue samples that were used for epigenetic analysis and levels of DNA-methylation (percent methylation) were quantified using targeted bisulfite sequencing. There were no differences among fish from contrasting ecotypes in methylation at heat shock protein 70 (HSP70) or heat shock protein 47 (HSP47), indicating that seasonal changes in DNA methylation may occur at finer spatial scales than at the level of ecotype. However, there was significant variation in DNA methylation level between montane populations at heat shock protein 47. These findings suggest that DNA methylation is unlikely to act as a source of plastic adaptive phenotypic variation in natural redband trout populations and therefore may not contribute greatly to thermal adaptation in coldwater fishes. Further research is needed to better inform whether and when DNA methylation might act as a of adaptive phenotypic variation, and more specifically, how population-level adaptation may influence epigenetic change in wild populations.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EData Use\u003C\/strong\u003E\u003Cbr \/\u003E\n\u003Cem\u003ELicense\u003C\/em\u003E: Attribution 4.0 International (\u003Ca href=\u0022https:\/\/creativecommons.org\/licenses\/by\/4.0\/legalcode\u0022\u003ECC-BY 4.0\u003C\/a\u003E)\u003Cbr \/\u003E\n\u003Cem\u003ERecommended Citation\u003C\/em\u003E:\u003Cbr \/\u003E\nKline BC, Williams BR, Loxterman JL, Keeley ER. 2023. Data from: DNA methylation of two heat-stress genes in redband trout from contrasting thermal environments [Dataset]. University of Idaho. \u003Ca href=\u0022https:\/\/doi.org\/10.7923\/gmx1-8g34\u0022\u003Ehttps:\/\/doi.org\/10.7923\/gmx1-8g34\u003C\/a\u003E\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EFunding\u003C\/strong\u003E\u003Cbr \/\u003E\nUS National Science Foundation and Idaho EPSCoR: \u003Ca href=\u0022https:\/\/www.nsf.gov\/awardsearch\/showAward?AWD_ID=1757324\u0022\u003EOIA-1757324\u003C\/a\u003E\u003C\/p\u003E\n","url":"https:\/\/data.nkn.uidaho.edu\/dataset\/data-dna-methylation-two-heat-stress-genes-redband-trout-contrasting-thermal-environments","state":"Active","log_message":"Update to resource Data Access | Data from: DNA methylation of two heat-stress genes in redband trout from contrasting thermal environments","private":true,"revision_timestamp":"Mon, 07\/08\/2024 - 11:10","metadata_created":"Thu, 09\/14\/2023 - 12:25","metadata_modified":"Mon, 07\/08\/2024 - 11:10","creator_user_id":"6cc16f2c-77c2-4d12-ac06-56bbb86b535b","type":"Dataset","resources":[{"id":"1d8b07ec-cf0a-4ddb-a627-5ce836cf57b2","revision_id":"","url":"https:\/\/www.northwestknowledge.net\/data\/240f0f19-3151-4779-9773-22bff99f6674","description":"","format":"data","state":"Active","revision_timestamp":"Mon, 07\/08\/2024 - 11:10","name":"Data Access | Data from: DNA methylation of two heat-stress genes in redband trout from contrasting thermal environments","mimetype":"data","size":"","created":"Thu, 09\/14\/2023 - 12:43","resource_group_id":"ea4b940c-347f-440a-ba90-23b6bb01560f","last_modified":"Date changed  Mon, 07\/08\/2024 - 11:10"},{"id":"c78496f2-41df-4d2f-aa50-c138574dd6e1","revision_id":"","url":"https:\/\/www.northwestknowledge.net\/data\/240f0f19-3151-4779-9773-22bff99f6674\/readme.txt","description":"","format":"txt","state":"Active","revision_timestamp":"Thu, 09\/14\/2023 - 12:52","name":"Metadata Access | Data from: DNA methylation of two heat-stress genes in redband trout from contrasting thermal environments (readme.txt)","mimetype":"txt","size":"","created":"Thu, 09\/14\/2023 - 12:44","resource_group_id":"ea4b940c-347f-440a-ba90-23b6bb01560f","last_modified":"Date changed  Thu, 09\/14\/2023 - 12:52"}],"tags":[{"id":"5f859265-7d19-47f5-a15e-0e22846d565a","vocabulary_id":"2","name":"adaptation"},{"id":"3c4b7b04-c8c8-46c4-bc38-4a5ad5c6e57a","vocabulary_id":"2","name":"climate change"},{"id":"2ddf177c-15c4-4755-a442-4d656453e890","vocabulary_id":"2","name":"common garden"},{"id":"425f965a-f839-486d-be20-95827298c8c0","vocabulary_id":"2","name":"ecology"},{"id":"bb2d577f-162f-43d8-bcc6-83dadb094c0a","vocabulary_id":"2","name":"epigenetics"},{"id":"87ac0d6d-729d-48c5-9f37-a8a40f41001c","vocabulary_id":"2","name":"gene\/genome\/genotype-environment interaction (GxE)"},{"id":"3bbad4f9-7651-4bf2-aacd-d11b259f3642","vocabulary_id":"2","name":"Oncorhynchus mykiss gairdneri"},{"id":"36a2c71f-ca58-468c-8c5d-ea3b9e8510c8","vocabulary_id":"2","name":"Redband Trout"},{"id":"78f28e49-0603-4e9b-baac-e2c0e6bd579c","vocabulary_id":"2","name":"phenotypic plasticity"},{"id":"435edef7-8f56-48e1-851a-1231e1537ef4","vocabulary_id":"2","name":"wildlife"},{"id":"323832b6-cd92-42c2-a030-1e27faaed762","vocabulary_id":"2","name":"thermal tolerance"},{"id":"1fdde502-4ae4-496a-87a3-dfb01f9fc0f7","vocabulary_id":"2","name":"heat shock protein"}],"groups":[{"description":"\u003Cp\u003EIdaho State University, a Carnegie-classified doctoral research and teaching institution founded in 1901, attracts students from around the world to its Idaho campuses. At the main campus in Pocatello, and at locations in Meridian, Idaho Falls and Twin Falls, ISU offers access to high-quality education in more than 250 programs.\u003C\/p\u003E\n\u003Cp\u003ELearn more at \u003Ca href=\u0022https:\/\/www.isu.edu\u0022\u003Ehttps:\/\/www.isu.edu\u003C\/a\u003E\u003C\/p\u003E\n","id":"ea4b940c-347f-440a-ba90-23b6bb01560f","image_display_url":"https:\/\/data.nkn.uidaho.edu\/sites\/default\/files\/ISU-black-transbkgrd.png","title":"Idaho State University","name":"group\/idaho-state-university"},{"description":"\u003Cp\u003EThe primary objective of Idaho EPSCoR is to stimulate research in niche areas that can become fully competitive in the disciplinary and multidisciplinary research programs of the National Science Foundation and other relevant agencies. Idaho EPSCoR provides support for sustainable increases in Research and Development capacity and advances science and engineering capabilities within the state. \u003C\/p\u003E\n\u003Cp\u003EVisit them at \u003Ca href=\u0022https:\/\/www.idahoepscor.org\u0022\u003Ehttps:\/\/www.idahoepscor.org\u003C\/a\u003E\u003C\/p\u003E\n","id":"e696b239-9ecb-412e-b032-03a75b2b9fd6","image_display_url":"https:\/\/data.nkn.uidaho.edu\/sites\/default\/files\/Idaho_epscor_logo_no_white_background.png","title":"Idaho EPSCoR","name":"group\/idaho-epscor"},{"description":"\u003Cp\u003EGEM3 is an NSF EPSCoR research program seeking to understand how genetic diversity and phenotypic plasticity affect species response to environmental change, shaping both population response and adaptive capacity.\u003C\/p\u003E\n\u003Cp\u003EVisit them at: \u003Ca href=\u0022https:\/\/www.idahogem3.org\u0022\u003Ehttps:\/\/www.idahogem3.org\u003C\/a\u003E\u003C\/p\u003E\n","id":"1812a312-11a4-492d-960b-9a78b4abfe0c","image_display_url":"https:\/\/data.nkn.uidaho.edu\/sites\/default\/files\/GEM3_nov5_logo.png","title":"EPSCoR GEM3","name":"group\/epscor-gem3"}]}]}<!DOCTYPE html PUBLIC "-//W3C//DTD XHTML 1.0 Strict//EN"
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