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GEM3 is an NSF EPSCoR research program seeking to understand how genetic diversity and phenotypic plasticity affect species response to environmental change, shaping both population response and adaptive capacity.

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Development of an In Vitro Method of Propagation for Basin Big Sagebrush (Artemisia tridentata subsp. tridentata)

Basin big sagebrush (Artemisia tridentata subsp. tridentata) is a keystone species of the sagebrush steppe, a widespread ecosystem of western North America threatened by climate change. The study’s goal was to develop an in vitro method of propagation for this taxon to support genome sequencing and genotype-by-environment research on drought tolerance. Such research may ultimately facilitate the reintroduction of big sagebrush in degraded habitats. Seedlings were generated from two diploid mother plants (2n = 2x = 18) collected in environments with contrasting precipitation regimes. The effects of IBA and NAA on rooting of shoot tips were tested on 45 individuals and 15 shoot tips per individual. Growth regulator and individual-seedling effects on percent rooting and roots per shoot tip were evaluated using statistical and clustering analyses. Furthermore, rooted shoot tips were transferred into new media to ascertain their continued growth in vitro. The results suggest that A. tridentata is an outbred species, as shown by individuals’ effect on rooting and growth. IBA addition was the most effective method for promoting adventitious rooting, especially in top-performing individuals. These individuals also have high survival and growth rates upon transferring to new media, making them suitable candidates for generating biomass for genome sequencing and producing clones for genotype-by-environment research.

Data and Resources

FieldValue
Modified
2021-02-26
Release Date
2021-02-17
Publisher
Homepage URL
Identifier
0d951448-423b-46c7-aa0e-7e65c0e247d3
Spatial / Geographical Coverage Area
POINT (-116.964 43.337)
POINT (-109.388 38.306)
Spatial / Geographical Coverage Location
Idaho and Utah (USA)
Temporal Coverage
Thursday, January 1, 2009 - 00:00 to Saturday, December 5, 2020 - 00:00
Language
English (United States)
License
Author
Rachael Barron, Peggy Martinez, Marcelo Serpe, Sven Buerki
Contact Name
Sven Buerki
Contact Email
Public Access Level
Public
Data available on:: 
Wednesday, February 17, 2021