Title: Data from: Video-rate raman-based metabolic imaging by airy light-sheet illumination and photon-sparse detection
	Dataset DOI: 10.11578/1908656

Data, code and/or products within this dataset support the follwoing manuscript: 
	Manuscript Title: Video-rate raman-based metabolic imaging by airy light-sheet illumination and photon-sparse detection 
	Journal: PNAS
	DOI: ### IN REVIEW

Description/Abstract:
	Data supporting manuscript submitted to PNAS: Video-Rate Raman-based Metabolic Imaging by Airy Light-Sheet Illumination and Photon-Sparse Detection. The data set includes: [1] raw data and [2] related images used in the analyses described within the manuscript.

	**Data Use**:
	*License*: [CC-BY 4.0 (https://creativecommons.org/licenses/by/4.0/)]
	*Recommended Citation*: Vasdekis AE (2023) Data from: Video-rate raman-based metabolic imaging by airy light-sheet illumination and photon-sparse detection [Dataset]. University of Idaho. https://doi.org/10.11578/1908656

Resource URL: https://data.nkn.uidaho.edu/dataset/data-video-rate-raman-based-metabolic-imaging-airy-light-sheet-illumination-and-photon

Creator(s):	
	1. Full Name: Andreas E. Vasdekis 
		Unique identifier: https://orcid.org/0000-0003-4315-1047
		Affiliation(s): University of Idaho

Other Contributor(s): NULL

Publisher: University of Idaho

Publication Year: 2023

Language(s): American English

Subject(s): 
	1. NATURAL SCIENCES
		1.3 Physical Science
		1.6 Biological Science

Keywords/Tags: Raman imaging, photon-sparse imaging, light-sheet microscopy

Resource Type General: Dataset

Dates: NULL	

Date available for the public: ### Pending release upon manuscript publication 

Sizes: 273.9 MB

Format(s): Compressed data deirectory (zip) with txt, csv and tiff files. 

Version: NULL

Funding References: 
	U.S. Department of Energy
		Award: DE-SC0022282
		URL: NULL
		Title: Integrative Imaging of Plant Roots during Symbiosis with Mycorrhizal Fungi

Spatial/Geographical Coverage Location: NULL

Temporal Coverage: NULL

Granularity of the Data: NULL

Contact Info:
	Contact Name: Andreas Vasdekis
	Contact Email: andreasv@uidaho.edu

Related Content:
	Peer-reviewed manuscript-PNAS | ### Pending link to manuscript
	Project associated code-GitHub | https://github.com/aevasdekis/photon-sparse#photon-sparse

Data Files: 

	Figure 4: file directory containing data files necessary to compile figure 4 of associated manuscript
		Figure_4a.csv: data for compiling the histogram presented in figure 4a 
			cell: ID of the cell observed
			sig_D2O: intensity values of a single cell grown in D2O
			bg_D2O: intensity values of background in D2O
			sig-bg_D2O: intensity difference between signal and background for a single cell grown in D2O
			sig_H2O: intensity values of a single cell grown in H2O [data only available for 1st 10 rows of data (cells h1-h10)]
			bg_H2O: intensity values of background in H2O [data only available for 1st 10 rows of data (cells h1-h10)]
			sig-bg_H2O: intensity difference between signal and background for a single cell grown in H2O [data only available for 1st 10 rows of data (cells h1-h10)]
		Figure_4b.csv: data for compiling the time-trace presented in figure 4b 
			time_min: temporal axis values (in sec)
			cell: intensity values for a single cell grown in D2O
			cell_norm: intensity values for a single cell grown in D2O, normalized at t = 1 min

	Figure 5: file directory containing data files necessary to compile figure 5 of associated manuscript
		220121_cloud_40x_90pct_focus.tif: 3D tif stack (xyt) representing single photon clouds as a function of time (t) of a single cell at focus
		Figure_5_error_analysis.csv: data of cell area error value represented in the insets of figure 5 
			photons-per-voxel: number of photons per unit voxel
			xy-area: cell area in the x-y plane in pixel units
			xy-area-per_norm: cell area in the x-y plane in pixel units normalized at 10 photons per voxel
			xz-area: cell area in the x-z plane in pixel units	
			xz-area-per_norm: cell area in the x-z plane in pixel units normalized at 10 photons per voxel

	Figure 6: file directory containing data files necessary to compile figure 6 of associated manuscript
	 	cell_1x1_figure_6a.tif: 2D tif image of a Y. lipolytica cell (as per Fig. 5) under pixel-level projection
		cell_2x2_figure_6b.tif: 2D tif image of a Y. lipolytica cell (as per Fig. 5) under sub-pixel projection
		Figure_6_traces.csv: data for compiling the traces presented in the insets of figure 6a and 6b
			Header Key:
				distance-pixel: x-axis distance in pixel units
				distance-μm: x-axis distance in micron units
				trace_1x1: trace of the Y. lipolytica cell under pixel-level projection
				trace_2x2: trace of the Y. lipolytica cell under sub-pixel projection