{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"28def103-1e65-4b1c-a44c-2346f62cf03d","name":"data-video-rate-raman-based-metabolic-imaging-airy-light-sheet-illumination-and-photon","title":"Data from: Video-rate raman-based metabolic imaging by airy light-sheet illumination and photon-sparse detection","author":"Andreas E. Vasdekis","author_email":"andreasv@uidaho.edu","maintainer":"RCDS Data Repository","maintainer_email":"rcds-web@uidaho.edu","license_title":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/","notes":"\u003Cp\u003EData supporting manuscript submitted to PNAS: Video-Rate Raman-based Metabolic Imaging by Airy Light-Sheet Illumination and Photon-Sparse Detection. The data set includes: [1] raw data and [2] related images used in the analyses described within the manuscript.\u003C\/p\u003E\n\u003Cp\u003EDespite its massive potential, Raman imaging represents just a modest fraction of all research and clinical microscopy to date. This is due to the ultralow Raman scattering cross-sections of most biomolecules that impose low-light or photon-sparse conditions. Bioimaging under such conditions is suboptimal, as it either results in ultralow frame rates or requires increased levels of irradiance. Here, we overcome this tradeoff by introducing Raman imaging that operates at both video rates and 1,000-fold lower irradiance than state-of-the-art methods. To accomplish this, we deployed a judicially designed Airy light-sheet microscope to efficiently image large specimen regions. Further, we implemented subphoton per pixel image acquisition and reconstruction to confront issues arising from photon sparsity at just millisecond integrations. We demonstrate the versatility of our approach by imaging a variety of samples, including the three-dimensional (3D) metabolic activity of single microbial cells and the underlying cell-to-cell variability. To image such small-scale targets, we again harnessed photon sparsity to increase magnification without a field-of-view penalty, thus, overcoming another key limitation in modern light-sheet microscopy.\u003C\/p\u003E\n\u003Cp\u003E\u003Cstrong\u003EData Use\u003C\/strong\u003E:\u003Cbr \/\u003E\n\u003Cem\u003ELicense\u003C\/em\u003E: \u003Ca href=\u0022https:\/\/creativecommons.org\/licenses\/by\/4.0\/\u0022\u003ECC-BY 4.0\u003C\/a\u003E\u003Cbr \/\u003E\n\u003Cem\u003ERecommended Citation\u003C\/em\u003E: Vasdekis AE (2023) Data from: Video-rate raman-based metabolic imaging by airy light-sheet illumination and photon-sparse detection [Dataset]. University of Idaho. \u003Ca href=\u0022https:\/\/doi.org\/10.11578\/1908656\u0022\u003Ehttps:\/\/doi.org\/10.11578\/1908656\u003C\/a\u003E\u003C\/p\u003E\n","url":"https:\/\/data.nkn.uidaho.edu\/dataset\/data-video-rate-raman-based-metabolic-imaging-airy-light-sheet-illumination-and-photon","state":"Active","log_message":"Update to resource Data Access | Video-rate raman-based metabolic imaging by airy light-sheet illumination and photon-sparse detection (compressed data directory)","private":true,"revision_timestamp":"Wed, 02\/22\/2023 - 11:31","metadata_created":"Tue, 12\/20\/2022 - 11:45","metadata_modified":"Wed, 02\/22\/2023 - 11:31","creator_user_id":"6cc16f2c-77c2-4d12-ac06-56bbb86b535b","type":"Dataset","resources":[{"id":"7f7822c1-8d2e-42e8-ad59-ad41ed37b6c1","revision_id":"","url":"https:\/\/data.nkn.uidaho.edu\/sites\/default\/files\/readme_15.txt","description":"","format":"txt","state":"Active","revision_timestamp":"Wed, 02\/22\/2023 - 11:29","name":"Metadata Access | Video-rate raman-based metabolic imaging by airy light-sheet illumination and photon-sparse detection (txt)","mimetype":"text\/plain","size":"5.98 KB","created":"Tue, 01\/10\/2023 - 14:32","resource_group_id":"89a02fc8-c98e-44bb-9d3e-2d3d0849fbca","last_modified":"Date changed Wed, 02\/22\/2023 - 11:29"},{"id":"8b718b11-9d5d-4b00-bade-29186c0cebd8","revision_id":"","url":"https:\/\/data.nkn.uidaho.edu\/sites\/default\/files\/Vasdekis_2023_RamanImaging_1.zip","description":"","format":"zip","state":"Active","revision_timestamp":"Wed, 02\/22\/2023 - 11:31","name":"Data Access | Video-rate raman-based metabolic imaging by airy light-sheet illumination and photon-sparse detection (compressed data directory)","mimetype":"application\/zip","size":"137.4 MB","created":"Wed, 02\/22\/2023 - 11:04","resource_group_id":"89a02fc8-c98e-44bb-9d3e-2d3d0849fbca","last_modified":"Date changed Wed, 02\/22\/2023 - 11:31"}],"tags":[{"id":"83af0da6-9ee1-49b9-83d7-7b68c8aa8a80","vocabulary_id":"2","name":"raman imaging"},{"id":"571a1ea5-bb71-44c2-ad96-1ca211f6cf81","vocabulary_id":"2","name":"photon-sparse imaging"},{"id":"de11d377-7e88-405b-883d-a1efc83f2cc3","vocabulary_id":"2","name":"light-sheet microscopy"}],"groups":[{"description":"\u003Cp\u003ESince 1889, the University of Idaho has provided motivated students with a transformative higher education experience that prepares them to solve real-world problems and achieve success in their lives and careers.\u003C\/p\u003E\n\u003Cp\u003ELearn more at \u003Ca href=\u0022https:\/\/www.uidaho.edu\u0022\u003Ehttps:\/\/www.uidaho.edu\u003C\/a\u003E\u003C\/p\u003E\n","id":"89a02fc8-c98e-44bb-9d3e-2d3d0849fbca","image_display_url":"https:\/\/data.nkn.uidaho.edu\/sites\/default\/files\/UI_Main_horizontal_4c.jpg","title":"University of Idaho","name":"group\/university-idaho"}]}]} Error | RCDS Data Repository

Error

The website encountered an unexpected error. Please try again later.