{"help":"Return the metadata of a dataset (package) and its resources. :param id: the id or name of the dataset :type id: string","success":true,"result":[{"id":"1660a1e4-4b2f-485c-80b6-7224d62e2061","name":"thesis-dna-methylation-heat-stress-genes-redband-trout-contrasting-thermal-enviornments","title":"Thesis | DNA Methylation of heat-stress genes in Redband Trout from contrasting thermal enviornments","author":"Benjamen Kline","author_email":"klineben@msu.edu","maintainer":"RCDS Data Repository","maintainer_email":"rcds-web@uidaho.edu","notes":"Epigenetic variation is a potential pathway for rapid response to environmental change and may be able to influence local adaptation at the population level through population by environment interactions. The most well-understood mechanism of epigenetic variation is DNA-methylation, a form of gene regulation that acts in response to environmental stress. Previous lab studies have shown ecotypic variation in DNA-methylation, but few have attempted to quantify epigenetic variation in natural populations. I developed a study to compare levels of DNA-methylation in redband trout (Oncorhynchus mykiss gairdneri) from contrasting environments. I sampled trout populations from cold montane and warm desert streams at repeated intervals, collecting tissue samples that were used for epigenetic analysis. Levels of DNA-methylation (percent methylation) were quantified using targeted bisulfite sequencing of two stress-related genes (Heat Shock Protein 70 and 47). There were no differences among fish from contrasting ecotypes, indicating that seasonal changes in DNA methylation may occur at finer spatial scales than at the level of ecotype. However, there were significant differences in DNA methylation among populations for heat shock protein 70 and between two montane populations for heat shock protein 47. These findings suggest that DNA methylation may act as a source of plastic adaptive phenotypic variation in natural populations and could be an important component of thermal adaptation in coldwater fishes. Further research is needed to better inform how DNA methylation acts as a source of adaptive phenotypic variation, and more specifically, how population-level adaptation may influence epigenetic change in wild populations.","url":"https:\/\/data.nkn.uidaho.edu\/dataset\/thesis-dna-methylation-heat-stress-genes-redband-trout-contrasting-thermal-enviornments","state":"Active","log_message":"Edited by awchild.","private":true,"revision_timestamp":"Thu, 07\/07\/2022 - 10:01","metadata_created":"Thu, 07\/07\/2022 - 09:58","metadata_modified":"Thu, 07\/07\/2022 - 10:01","creator_user_id":"6cc16f2c-77c2-4d12-ac06-56bbb86b535b","type":"Dataset","tags":[{"id":"5f859265-7d19-47f5-a15e-0e22846d565a","vocabulary_id":"2","name":"adaptation"},{"id":"3c4b7b04-c8c8-46c4-bc38-4a5ad5c6e57a","vocabulary_id":"2","name":"climate change"},{"id":"4ff8f12f-597f-460d-98ea-6650f2124e30","vocabulary_id":"2","name":"environmental change"},{"id":"bb2d577f-162f-43d8-bcc6-83dadb094c0a","vocabulary_id":"2","name":"epigenetics"},{"id":"78f28e49-0603-4e9b-baac-e2c0e6bd579c","vocabulary_id":"2","name":"phenotypic plasticity"},{"id":"42757e2d-85a6-4372-b505-bc252b94da2a","vocabulary_id":"2","name":"DNA methlyation"},{"id":"323832b6-cd92-42c2-a030-1e27faaed762","vocabulary_id":"2","name":"thermal tolerance"},{"id":"1fdde502-4ae4-496a-87a3-dfb01f9fc0f7","vocabulary_id":"2","name":"heat shock protein"},{"id":"884eefe2-190a-4560-ad43-2ef8d23ecb7b","vocabulary_id":"2","name":"desert"},{"id":"86973268-fdaf-49d0-98c9-26bb34f821c2","vocabulary_id":"2","name":"montane"}],"groups":[{"description":"\u003Cp\u003EIdaho State University, a Carnegie-classified doctoral research and teaching institution founded in 1901, attracts students from around the world to its Idaho campuses. At the main campus in Pocatello, and at locations in Meridian, Idaho Falls and Twin Falls, ISU offers access to high-quality education in more than 250 programs.\u003C\/p\u003E\n\u003Cp\u003ELearn more at \u003Ca href=\u0022https:\/\/www.isu.edu\u0022\u003Ehttps:\/\/www.isu.edu\u003C\/a\u003E\u003C\/p\u003E\n","id":"ea4b940c-347f-440a-ba90-23b6bb01560f","image_display_url":"https:\/\/data.nkn.uidaho.edu\/sites\/default\/files\/ISU-black-transbkgrd.png","title":"Idaho State University","name":"group\/idaho-state-university"},{"description":"\u003Cp\u003EThe primary objective of Idaho EPSCoR is to stimulate research in niche areas that can become fully competitive in the disciplinary and multidisciplinary research programs of the National Science Foundation and other relevant agencies. Idaho EPSCoR provides support for sustainable increases in Research and Development capacity and advances science and engineering capabilities within the state. \u003C\/p\u003E\n\u003Cp\u003EVisit them at \u003Ca href=\u0022https:\/\/www.idahoepscor.org\u0022\u003Ehttps:\/\/www.idahoepscor.org\u003C\/a\u003E\u003C\/p\u003E\n","id":"e696b239-9ecb-412e-b032-03a75b2b9fd6","image_display_url":"https:\/\/data.nkn.uidaho.edu\/sites\/default\/files\/Idaho_epscor_logo_no_white_background.png","title":"Idaho EPSCoR","name":"group\/idaho-epscor"},{"description":"\u003Cp\u003EGEM3 is an NSF EPSCoR research program seeking to understand how genetic diversity and phenotypic plasticity affect species response to environmental change, shaping both population response and adaptive capacity.\u003C\/p\u003E\n\u003Cp\u003EVisit them at: \u003Ca href=\u0022https:\/\/www.idahogem3.org\u0022\u003Ehttps:\/\/www.idahogem3.org\u003C\/a\u003E\u003C\/p\u003E\n","id":"1812a312-11a4-492d-960b-9a78b4abfe0c","image_display_url":"https:\/\/data.nkn.uidaho.edu\/sites\/default\/files\/GEM3_nov5_logo.png","title":"EPSCoR GEM3","name":"group\/epscor-gem3"}]}]} Error | RCDS Data Repository

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